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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/07/2013 |
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Data da última atualização: |
16/07/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUEZ, M. A. D.; HERAI, R.; WAALWIJK, C.; YAMAGISHI, M. E. B.; GIACHETTO, P. F.; FERREIRA, G. B.; SOUZA, M.; KEMA, G. H. J. |
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Afiliação: |
MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; R. HERAI, UNICAMP; C. WAALWIJK, Plant Research International B. V.; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; GILVAN BARBOSA FERREIRA, CNPA; M. SOUZA; G.H.J. KEMA, Plant Research International B. V. |
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Título: |
Comparative transcriptome analyses and genome assembly of fusarium oxysporum f. sp. cubense. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Acta Horticulturae, n. 986, p. 165-168, 2013. |
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Páginas: |
4p. |
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ISSN: |
0567-7572 |
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Idioma: |
Inglês |
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Notas: |
Trabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. |
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Conteúdo: |
Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana, is a highly destructive and genetically diverse pathogen. Despite its economic importance, genomic information about Foc is limited and no transcriptomic analyses have been reported so far. By using 454 sequencing technology, we generated >2.5 million expressed sequenced tags (ESTs) from four Foc isolates representing different vegetative compatibility groups (VCGs) and races that infect banana: race 1 (R1, VCG unknown but different from the others here described), race 2 (R2, VCG 0124), subtropical race 4 (SR4, VCG 0120) and tropical race 4 (TR4, VCG 01213). The ESTs were obtained from libraries prepared from mRNA extracted from three physiological states (mycelium, conidia and germinated conidia), which were pooled in a 2:2:1 ratio. Most genes are represented in all libraries, but in silico comparative analyses identified a set of unique ESTs for each isolate (689 for R1, 974 for R2, 296 for SR4 and 555 for TR4), which are excellent candidates for diagnostics development, future plant-pathogen interaction studies and functional analyses. In subsequent analyses, a 40× sequencing-coverage (Illumina single reads) of TR4 genomic DNA was assembled in a de novo based methodology, resulting in a 45.5 Mb genome. Preliminary analyses show a high colinearity of EST and genomic data that significantly contributes to the quality of the assembly. |
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Thesagro: |
Banana; Fusariose; Fusarium. |
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Thesaurus Nal: |
Bananas; Fusarium oxysporum f. sp. cubense; Fusarium wilt; Genome. |
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Categoria do assunto: |
-- |
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Marc: |
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245 $aComparative transcriptome analyses and genome assembly of fusarium oxysporum f. sp. cubense.
260 $c2013
300 $a4p.
500 $aTrabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses.
520 $aFusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana, is a highly destructive and genetically diverse pathogen. Despite its economic importance, genomic information about Foc is limited and no transcriptomic analyses have been reported so far. By using 454 sequencing technology, we generated >2.5 million expressed sequenced tags (ESTs) from four Foc isolates representing different vegetative compatibility groups (VCGs) and races that infect banana: race 1 (R1, VCG unknown but different from the others here described), race 2 (R2, VCG 0124), subtropical race 4 (SR4, VCG 0120) and tropical race 4 (TR4, VCG 01213). The ESTs were obtained from libraries prepared from mRNA extracted from three physiological states (mycelium, conidia and germinated conidia), which were pooled in a 2:2:1 ratio. Most genes are represented in all libraries, but in silico comparative analyses identified a set of unique ESTs for each isolate (689 for R1, 974 for R2, 296 for SR4 and 555 for TR4), which are excellent candidates for diagnostics development, future plant-pathogen interaction studies and functional analyses. In subsequent analyses, a 40× sequencing-coverage (Illumina single reads) of TR4 genomic DNA was assembled in a de novo based methodology, resulting in a 45.5 Mb genome. Preliminary analyses show a high colinearity of EST and genomic data that significantly contributes to the quality of the assembly.
650 $aBananas
650 $aFusarium oxysporum f. sp. cubense
650 $aFusarium wilt
650 $aGenome
650 $aBanana
650 $aFusariose
650 $aFusarium
700 1 $aHERAI, R.
700 1 $aWAALWIJK, C.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aGIACHETTO, P. F.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 41 | |
| 1. |  | DITA, M. A.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, v. 34, p. S177, ago. 2009. Suplemento. Ref. 606. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
|    |
| 2. | | KOBAYASHI, A. K.; RODRIGUEZ, M. A. D.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Generation of Fusarium f. sp. passiflorae strains expressing reporter genes. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 607.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | DITA, M. A.; HERAI, R.; WAALWIJK, C.; YAMAGISHI, M.; GIACHETTO, P.; FERREIRA, G.; SOUZA, M.; KEMA, G. H. J. Comparative transcriptome analysis and genome assembly of Fusarium oxysporum f. sp. cubense. In: INTERNATIONAL PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [S.l.]: ISHS, 2011. p. 58.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
|  |
| 8. | | WAALWIJK, C.; DITA, M. A.; BUDDENHAGEN, I.; PAIVA, L. V. K.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Development of a detection method for tropical race 4 of Fusarium oxysporum f. sp. cubense. In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p.38.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | DÍAZ-TRUJILLO, C.; KOBAYASHI, A. K.; ZWIERS, L. H; SOUZA JÚNIOR, M. T.; KEMA, G. H. J. Developing tools for Mycosphaerella fijiensis studies. Gewasbescherming, v. 40, n. 3, p. 129, mei 2009. Edição do Fast Firward 40 jaar Gewasbescherming, 2009, Wageningen.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 10. | | RODRIGUEZ, M. A. D.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 606.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | KOBAYASHI, A. K.; DITA, M. A.; TROCOLI, R. O.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. GFP and RFP transformation of Fusarium guttiforme (sin. Fusarium subglutinans f. sp. annanas). Tropical Plant Pathology, v. 34, p. S156, ago. 2009. Suplemento. Ref. 545. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. A greenhouse bioassay for the Fusarium oxysporum f. sp. cubense x ?Grand Naine? (Musa, AAA, Cavendish Subgroup) interaction. Acta Horticulturae, v. 897, p. 377-380, may 2011.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | DÍAZ-TRUJILLO, C.; FORTES, C. F.; CAPDEVILLE, G. de; JALINK, H.; SOUZA, M.; KEMA, G. H. J. A reliable routine assay for black leaf streak disease of bananas caused by Mycosphaerella fijiensis. In: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburgh, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | MUNIZ, C. R.; SILVA, G. F. da; SOUZA JUNIOR, M. T.; FREIRE, F. das C. O.; KEMA, G. H. J.; GUEDES, M. I. F. Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants. Genetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Amazônia Ocidental. |
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| 17. | | RODRIGUEZ, M. A. D.; HERAI, R.; WAALWIJK, C.; YAMAGISHI, M. E. B.; GIACHETTO, P. F.; FERREIRA, G. B.; SOUZA, M.; KEMA, G. H. J. Comparative transcriptome analyses and genome assembly of fusarium oxysporum f. sp. cubense. Acta Horticulturae, n. 986, p. 165-168, 2013. 4p. Trabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 18. | | RODRIGUEZ, M. A. D.; HERAI, R.; WAALWIJK, C.; YAMAGISHI, M. E. B.; GIACHETTO, P. F.; FERREIRA, G.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Comparative transcriptome analysis and genome assembly of Fusarium oxysporum f. sp. cubense. In: ISHS / PROMUSA SYMPOSIUM, 2011, Salvador, BH. Bananas and plantains: toward sustainable global production and improved uses: program and abstracts. Leuven: ISHS, 2011. p. 58| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; DIAZ, C.; D'HONT, A.; YAHIAOUE, N.; BAKRLY, F.; SOUZA JR. M.; KEMA, G. H. J. Characterization of Musa germplasm for resistance to tropical race 4 of fusarium oxysporum f. sp. cubense. In: PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [Montepellier.]: [Bioversity International, 2011. p.37.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 20. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; DIAZ, C.; D´HON, A.; YAHIAOUI, N.; CARREEL, F.; BAKRY, F.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Characterization of Musa germplasm for resistance to tropical race 4 of Fusarium oxysporum f. sp. cubense. In: ISHS / PROMUSA SYMPOSIUM, 2011, Salvador, BH. Bananas and plantains: toward sustainable global production and improved uses: program and abstracts. Leuven: ISHS, 2011. p. 49| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 41 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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